Flow Cytometry Core

What do we do?

The Children’s Research Institute and Cancer Center Flow Cytometry Shared Resource is an advanced technology facility primarily serving MCW and CRI investigators. The facility provides secondary support to on-campus colleagues at the Versiti-BRI, as well as collaborators off campus at institutions in the Upper Midwest. Our facility provides 24/7/365 access to analytical cytometers and cell sorters for trained users and operator-assisted cell sorting by appointment during business hours. In addition to these services, we assist both new and established investigators with protocol and assay development, including sample preparation, selection of antibodies and fluorochromes, proper staining and compensation controls, and selection of appropriate buffers. Assistance with Data analysis using FlowJo, FCS Express, DIVA and SpectroFlo software is also available by appointment.

What can flow cytometry do for you?

You can study:

• Cellular phenotype and functional responses
• Cell cycle and apoptosis
• Cell signaling and transcriptional regulation

What can be analyzed by flow cytometry?

• Blood cells
• Primary tissue cells
• Stem cells (e.g., bone marrow)
• Bacteria and protozoa
• Nanoparticles (e.g., EV and exosomes)

Equipment available

• BD LSRFortessa X20 – 5-laser (18 colors, 20 parameters) flow cytometer
• 2 Cytek Aurora – 5-laser (64 colors, 67 parameters) flow cytometers
• Cytek Aurora CS – 5-laser (64 colors, 67 parameters) cell sorter
• BD FACSAria II – 4-laser (10 colors, 12 parameters) cell sorter
• NanoCELECT WOLF-Sorter – 1-laser (3 colors, 5 parameters) cell sorter
• Miltenyi MACSQuant Tyto – 3-laser (8 colors, 10 parameters) cell sorter
• HemaVET 950 - CBC analyzer (24 parameters, including platelets; multi-species)
• DeNovix CellDrop FL
• FlowJo software
• FCS express software
• SpectroFlo software   

Contact us

Calvin B. Williams, MD, PhD
Director: cbwillia@mcw.edu

Galina Petrova, PhD
Manager: gpetrova@mcw.edu | (414) 955-5793 | Monday – Friday, 9:00 a.m. – 5:00 p.m.

MACC Fund Research Center, 5th Floor, Suite 5052
Medical College of Wisconsin, 8701 W Watertown Plank Road, Milwaukee, Wisconsin, 53226